Journal: bioRxiv

Article Title: Endothelial Heterogeneity Across Vascular Beds Impacts Inflammatory Signaling and Neutrophil Adhesion

doi: 10.64898/2026.05.26.727909

Figure Lengend Snippet: Multiplexed ELISA of conditioned media from unstimulated umbilical artery (HUAEC), umbilical vein (HUVEC), dermal microvascular (HDMEC), and pulmonary microvascular (HPMEC) endothelial cells. Conditioned media was collected from 1 well per cell type after 16 hours incubation. Samples were analyzed with a Luminex Magpix device and a custom ProcartaPlex inflammation panel. Data are represented as (A) Heatmap normalized across the range for each protein, with darker tones corresponding to higher expression and less expression for lighter tones. Factors that measured below the limit of detection are indicated with an X. (B) log 10 (average expression) ± SEM for N=4 independent experiments. Factors that measured below the limit of detection are indicated with an X. Full statistical analysis with one-way ANOVA and Tukey-Kramer pairwise comparisons testing was done for average expression across all cell types, with results presented in Supplemental Figure 1.

Article Snippet: The following cells were used: pooled human umbilical vein endothelial cells (HUVEC, PromoCell C-12203, Heidelberg, Germany), human umbilical arterial endothelial cells (HUAEC, PromoCell C-12202, Heidelberg, Germany), adult dermal microvascular endothelial cells (HDMEC, PromoCell C-12212, Heidelberg, Germany), and adult pulmonary microvascular endothelial cells (HPMEC, PromoCell C-12281, Heidelberg, Germany).

Techniques: Enzyme-linked Immunosorbent Assay, Incubation, Luminex, Expressing

Journal: bioRxiv

Article Title: Endothelial Heterogeneity Across Vascular Beds Impacts Inflammatory Signaling and Neutrophil Adhesion

doi: 10.64898/2026.05.26.727909

Figure Lengend Snippet: Multiplexed ELISA of conditioned media from TNF-stimulated (50ng/mL TNF in media) umbilical artery (HUAEC), umbilical vein (HUVEC), dermal microvascular (HDMEC), and pulmonary microvascular (HPMEC) endothelial cells. Conditioned media was collected from 1 well per cell type after 16 hours incubation. Samples were analyzed with a Luminex Magpix device and a custom ProcartaPlex inflammation panel. (A) Heatmap normalized across the range for each protein, with darker tones corresponding to higher expression and less expression for lighter tones. (B) Fold-change expression differences from unstimulated control for factors with >8 fold increase by at least one cell type. Data are average fold change-expression for N=4 independent experiments. One-way ANOVA with Tukey-Kramer pairwise comparisons testing was done for each condition, with asterisks indicating statistical significance between conditions (*p<0.05).

Article Snippet: The following cells were used: pooled human umbilical vein endothelial cells (HUVEC, PromoCell C-12203, Heidelberg, Germany), human umbilical arterial endothelial cells (HUAEC, PromoCell C-12202, Heidelberg, Germany), adult dermal microvascular endothelial cells (HDMEC, PromoCell C-12212, Heidelberg, Germany), and adult pulmonary microvascular endothelial cells (HPMEC, PromoCell C-12281, Heidelberg, Germany).

Techniques: Enzyme-linked Immunosorbent Assay, Incubation, Luminex, Expressing, Control

Journal: bioRxiv

Article Title: Endothelial Heterogeneity Across Vascular Beds Impacts Inflammatory Signaling and Neutrophil Adhesion

doi: 10.64898/2026.05.26.727909

Figure Lengend Snippet: Multiplexed ELISA of conditioned media from P. aeuriginosa -stimulated (0.05 OD in media) umbilical artery (HUAEC), umbilical vein (HUVEC), dermal microvascular (HDMEC), and pulmonary microvascular (HPMEC) endothelial cells. Conditioned media was collected from 1 well per cell type after 16 hours incubation. Samples were analyzed with a Luminex Magpix device and a custom ProcartaPlex inflammation panel. (A) Heatmap normalized across the range for each protein, with darker tones corresponding to higher expression and less expression for lighter tones. (B) Fold-change expression differences from unstimulated control for factors with >8 fold increase by at least one cell type. Data are average fold change-expression for N=4 independent experiments. One-way ANOVA with Tukey-Kramer pairwise comparisons testing was done for each condition, with asterisks indicating statistical significance between conditions (*p<0.05).

Article Snippet: The following cells were used: pooled human umbilical vein endothelial cells (HUVEC, PromoCell C-12203, Heidelberg, Germany), human umbilical arterial endothelial cells (HUAEC, PromoCell C-12202, Heidelberg, Germany), adult dermal microvascular endothelial cells (HDMEC, PromoCell C-12212, Heidelberg, Germany), and adult pulmonary microvascular endothelial cells (HPMEC, PromoCell C-12281, Heidelberg, Germany).

Techniques: Enzyme-linked Immunosorbent Assay, Incubation, Luminex, Expressing, Control

Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

Article Title: Activin A–Endothelin-1 Axis Governs Pulmonary Vascular Remodeling: Mechanistic Basis for Emerging Therapies in PAH

doi: 10.1161/ATVBAHA.125.323681

Figure Lengend Snippet: In vivo activin A inhibition improves pulmonary hypertension (PH) phenotype comparably or more than ET-1 (endothelin-1) blockade. A , Experimental design: wild-type (WT) and VE-cadherin (vascular endothelial cadherin)–INHBA (inhibin β-A)-Tg (TG/transgenic) mice were exposed to hypoxia (10% O 2 ) for 3 weeks, with vehicle (VEH), FST (follistatin; 8.5 μg/kg), bosentan (BOS; 30 mg/kg), or FST+BOS administered during the final 2 weeks. B , Right ventricular systolic pressure (RVSP; n=4–9). C , Fulton index (RV/[LV+S] [right ventricle to left ventricle plus septum] ratio; n=4–8). D , Representative hematoxylin and eosin–stained lung sections. Blue arrows indicate vessels. E , Representative immunofluorescent staining of α-SMA (α-smooth muscle actin protein; green, SMC [smooth muscle cell] marker), vWF (von Willebrand Factor; red, endothelial cell [EC] marker), and DAPI (4′,6-diamidino-2-phenylindole; blue, nuclei). White arrows indicate vessels. F , Quantification of pulmonary artery muscularization (non-, partial-, full; n=12–15 fields from 3–4 mice). PA indicates pulmonary artery. G , Lung ET-1 mRNA expression (n=3–4). H , mRNA expression of INHBA, ET-1, eNOS (endothelial NO synthase), SOD2 (superoxide dismutase 2), fibronectin, SLUG (snail family transcriptional repressor 2), CD31 (cluster of differentiation 31), and BMP4 (bone morphogenetic protein 4) in lung ECs isolated from WT and TG mice (n=3–4). Data are mean±SEM. P <0.05 is deemed statistically significant. Tests: 1-way ANOVA with Tukey post hoc test for B , C , and G ; 2-way ANOVA with Tukey post hoc test for F ; 2-sided Student t test for H .

Article Snippet: Human pulmonary artery ECs (PAECs; no. C-12241; PromoCell) and pulmonary artery smooth muscle cells (PASMCs; no. C-12521; PromoCell) were cultured in HuMedia-EG2 (Kurabo) and smooth muscle cells growth medium 2 (PromoCell), respectively, at 37 °C and 5% CO 2 .

Techniques: In Vivo, Inhibition, Transgenic Assay, Staining, Marker, Expressing, Isolation